Journal: Materials Today Bio

Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions

doi: 10.1016/j.mtbio.2026.103015

Figure Lengend Snippet: Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Following the 30 - min incubation in the dark, images were collected with the ZEISS inverted fluorescence microscope (Carl Zeiss AG, Oberkochen, Germany; Model: Axio Observer 7).

Techniques: Fourier Transform Infrared Spectroscopy, Spectroscopy, Imaging, Microscopy, Pore Size, Fluorescence, Co-Culture Assay, Cell Culture

Journal: Colloids and surfaces. B, Biointerfaces

Article Title: Novel hydrogel-based cancer-on-a-chip models for growth of 3D multi-cellular structures and investigation of early angiogenesis in pancreatic ductal adenocarcinoma.

doi: 10.1016/j.colsurfb.2025.114736

Figure Lengend Snippet: Fig. 1. A) Visual scheme of the fabrication protocols for producing the hydrogels of this study. Created with BioRender. B) Schematic of the microfluidic devices used in this study. Hydrogels and PANC-1 cells are introduced through the loading ports (2) into the central chambers of the devices (1). Culture medium is introduced through the reservoirs (3). C) Representative brightfield microscopy images of the two types of PANC-1 3D multi-cellular structures observed in this study inside microfluidic devices: spheroids (red arrows), with an inner lumen delimited by a cell ring, and cell aggregates (yellow arrows), with cells arranging in grape-like clusters. D) Schematic illustration showing the difference between spheroids and cell aggregates. Created with BioRender.

Article Snippet: Multi-cellular structures formation and growth in microfluidic devices (N = 3 per condition) was observed and photographed by brightfield microscopy (DM IL LED, Leica) over 14 days of culture.

Techniques: Microscopy

Journal: Colloids and surfaces. B, Biointerfaces

Article Title: Novel hydrogel-based cancer-on-a-chip models for growth of 3D multi-cellular structures and investigation of early angiogenesis in pancreatic ductal adenocarcinoma.

doi: 10.1016/j.colsurfb.2025.114736

Figure Lengend Snippet: Fig. 3. Brightfield microscopy images of PANC-1 3D multi-cellular structures growth in hydrogels loaded inside one-chamber microfluidic devices: low cell density cultures. Images show the central chamber of the microfluidic device. Scale bar = 175 µm.

Article Snippet: Multi-cellular structures formation and growth in microfluidic devices (N = 3 per condition) was observed and photographed by brightfield microscopy (DM IL LED, Leica) over 14 days of culture.

Techniques: Microscopy

Journal: Colloids and surfaces. B, Biointerfaces

Article Title: Novel hydrogel-based cancer-on-a-chip models for growth of 3D multi-cellular structures and investigation of early angiogenesis in pancreatic ductal adenocarcinoma.

doi: 10.1016/j.colsurfb.2025.114736

Figure Lengend Snippet: Fig. 4. Brightfield microscopy images of PANC-1 3D multi-cellular structures growth in hydrogels loaded inside one-chamber microfluidic devices: high cell density cultures. Images show the central chamber of the microfluidic device. Red arrows show displacement of the collagen I hydrogel. Scale bar = 175 µm.

Article Snippet: Multi-cellular structures formation and growth in microfluidic devices (N = 3 per condition) was observed and photographed by brightfield microscopy (DM IL LED, Leica) over 14 days of culture.

Techniques: Microscopy

Journal: Colloids and surfaces. B, Biointerfaces

Article Title: Novel hydrogel-based cancer-on-a-chip models for growth of 3D multi-cellular structures and investigation of early angiogenesis in pancreatic ductal adenocarcinoma.

doi: 10.1016/j.colsurfb.2025.114736

Figure Lengend Snippet: Fig. 8. A) Visual schematic of the experiment. B) Representative brightfield microscopy images of control cultures and co-cultures on days 1 and 3 of culture. C) Representative fluorescence microscopy images of co-cultures in two-chamber microfluidic devices on day 3 where the blue fluorescence is due to DAPI-stained dsDNA in the cells nuclei and the orange fluorescence is due to phalloidin-stained actin filaments in the cytoskeleton of cells. For both microenvironments, cells can be seen at different z-planes.

Article Snippet: Multi-cellular structures formation and growth in microfluidic devices (N = 3 per condition) was observed and photographed by brightfield microscopy (DM IL LED, Leica) over 14 days of culture.

Techniques: Microscopy, Control, Fluorescence, Staining